Neurobiology of Disease -Synuclein Overexpression in PC12 and Chromaffin Cells Impairs Catecholamine Release by Interfering with a Late Step in Exocytosis
نویسندگان
چکیده
Kristin E. Larsen,1* Yvonne Schmitz,1* Matthew D. Troyer,5 Eugene Mosharov,1 Paula Dietrich,1 Abrar Z. Quazi,6 Magali Savalle,1 Venu Nemani,5 Farrukh A. Chaudhry,6 Robert H. Edwards,5 Leonidas Stefanis,1,2 and David Sulzer1,3,4 Departments of 1Neurology, 2Pathology, and 3Psychiatry and Pharmacology, Columbia University School of Medicine, New York, New York 10032, 4Department of Neuroscience, New York Psychiatric Institute, New York, New York 10032, 5Departments of Neurology and Physiology, University of California School of Medicine, San Francisco, San Francisco, California 94143, and 6Biotechnology Centre of Oslo and Centre for Molecular Biology and Neuroscience, University of Oslo, N-0317, Oslo, Norway
منابع مشابه
Further characterization of dopamine release by permeabilized PC12 cells.
Rat pheochromocytoma cells (PC12) permeabilized with staphylococcal alpha-toxin release [3H]dopamine after addition of micromolar Ca2+. This does not require additional Mg2+-ATP (in contrast to bovine adrenal medullary chromaffin cells). We also observed Ca2+-dependent [3H]-dopamine release from digitonin-permeabilized PC12 cells. Permeabilization with alpha-toxin or digitonin and stimulation o...
متن کاملIntroduction of macromolecules into bovine adrenal medullary chromaffin cells and rat pheochromocytoma cells (PC12) by permeabilization with streptolysin O: inhibitory effect of tetanus toxin on catecholamine secretion.
Conditions are described for controlled plasma membrane permeabilization of rat pheochromocytoma cells (PC12) and cultured bovine adrenal chromaffin cells by streptolysin O (SLO). The transmembrane pores created by SLO invoke rapid efflux of intracellular 86Rb+ and ATP, and also permit passive diffusion of proteins, including immunoglobulins, into the cells. SLO-permeabilized PC12 cells release...
متن کاملThe anti-fibrinolytic SERPIN, plasminogen activator inhibitor 1 (PAI-1), is targeted to and released from catecholamine storage vesicles.
Recent studies suggest a crucial role for plasminogen activator inhibitor-1 (PAI-1) in mediating stress-induced hypercoagulability and thrombosis. However, the mechanisms by which PAI-1 is released by stress are not well-delineated. Here, we examined catecholaminergic neurosecretory cells for expression, trafficking, and release of PAI-1. PAI-1 was prominently expressed in PC12 pheochromocytoma...
متن کاملArachidonic acid release and catecholamine secretion from digitonin-treated chromaffin cells: effects of micromolar calcium, phorbol ester, and protein alkylating agents.
The relationship between catecholamine secretion and arachidonic acid release from digitonin-treated chromaffin cells was investigated. Digitonin renders permeable the plasma membranes of bovine adrenal chromaffin cells to Ca2+, ATP, and proteins. Digitonin-treated cells undergo exocytosis of catecholamine in response to micromolar Ca2+ in the medium. The addition of micromolar Ca2+ to digitoni...
متن کاملComparison of Cysteine String Protein (Csp) and Mutant a-SNAP Overexpression Reveals a Role for Csp in Late Steps of Membrane Fusion in Dense-Core Granule Exocytosis in Adrenal Chromaffin Cells
Assembly of the SNARE complex and its disassembly caused by the action of soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) and NSF is crucial for the maintenance of vesicular traffic, including fusion of regulated exocytotic vesicles. Various other proteins may also have important roles in the processes leading to membrane fusion via interaction with the SNARE proteins,...
متن کامل